کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7229741 1470928 2018 26 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ultrasensitive detection of lysozyme in droplet-based microfluidic devices
ترجمه فارسی عنوان
تشخیص حساسیت از لیزوزیم در دستگاه های میکرو فلوئیدیک مبتنی بر قطره
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Lysozyme (LYS) is a bacteriolytic enzyme, available in secretions such as saliva, tears and human milk. LYS is an important defence molecule of the innate immune system, and its overexpression can be a consequence of diseases such as leukemia, kidney disease and sarcoidosis. This paper reports on a digital microfluidic-based approach that combines the gold nanoparticle-enhanced chemiluminescence with aptamer interaction to detect human lysozyme into droplets 20 nanoliters in volume. The described method allows identifying LYS with a 44.6 femtomolar limit of detection, using sample volume as low as 1 μL and detection time in the range of 10 min. We used luminol to generate the chemiluminescence and demonstrated that the compartmentalization of LYS in droplets also comprising gold nanoparticles provided enhanced luminescence. We functionalized the gold nanoparticles with a thiolated aptamer to achieve the required selectivity that allowed us to detect LYS in human serum.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 104, 1 May 2018, Pages 8-14
نویسندگان
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