کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7229978 1470930 2018 29 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Graphene and Au NPs co-mediated enzymatic silver deposition for the ultrasensitive electrochemical detection of cholesterol
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Graphene and Au NPs co-mediated enzymatic silver deposition for the ultrasensitive electrochemical detection of cholesterol
چکیده انگلیسی
Cholesterol is an essential ingredient in mammals, and serum cholesterol is a major component of atherosclerotic plaques. The level of cholesterol in human serum has become an important index for clinical diagnosis and prevention of cardiovascular disease. In this paper, a simple and ultrasensitive cholesterol biosensor based on graphene oxide (GO) and gold nanoparticles (Au NPs) co-mediated enzymatic silver deposition was designed by immobilizing cholesterol oxidase (CHOD), cholesterol esterase (CHER) and GO onto the surface of Au NPs modified screen-printed carbon electrode (SPE). Under the synergistic effect of CHER, CHOD and GO, the cholesterol was hydrolyzed to generate hydrogen peroxide, which can reduce the silver (Ag) ions in the solution to metallic Ag which deposited on the surface of Au NPs modified SPE. The ultrasensitive detection of cholesterol was achieved by anodic stripping voltammetry measurement of the enzymatically deposited Ag. Under optimal conditions, the anodic stripping peak current of Ag increased with the increasing cholesterol concentration in the range from 0.01 μg/mL to 5000 μg/mL with a limit of detection of 0.001 μg/mL (S/N = 3). In addition, the ultrasensitive cholesterol biosensor exhibited higher specificity, acceptable reproducibility and excellent recoveries for cholesterol detection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 102, 15 April 2018, Pages 560-567
نویسندگان
, , , , , , , , , , , , , , ,