کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230123 | 1470944 | 2017 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Label-free ratiometric DNA detection using two kinds of interaction-responsive emission dyes
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A novel ratiometric method with label-free and dual-wavelength signal outputs was developed for DNA detection by employing two kinds of structure-specific dyes that characterising interaction-responsive emission property. The ratiometric platform for the fluorescent detection of DNA was based on target-induced structural transformation from stem-loop structure incorporating split G-quadruplex (SISG) to doubule-strand DNA (dsDNA). The SISG conformation will be disrupted upon binding to the target DNA of perfect complementary sequence, resulting in fluorescence decrease at 610Â nm as NMM releases from SISG and fluorescence increase at 450Â nm as DAPI inserts into the dsDNA. The method demonstrated its simplicity in that it saved the trouble of expensive and cumbersome functionalization process compared with reported ratiometric methods, and that the two dyes could be excited at the same excitation wavelength. More intriguingly, the combinatorial employment of the two kinds of structure-selective dyes demonstrated a result of “heterosis”, in that this sensor simultaneously inherits the sensitivity of DAPI-based signal and the selectivity of G4-based signal. Besides that, this proposed biosensor has been successfully applied in serum sample for DNA detection, and provided a simple and sensitive method for potential DNA detection in bioanalysis, food analysis and disease diagnostic.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 87, 15 January 2017, Pages 320-324
Journal: Biosensors and Bioelectronics - Volume 87, 15 January 2017, Pages 320-324
نویسندگان
Yahui Guo, Qingmin Chen, Yiting Qi, Yunfei Xie, He Qian, Weirong Yao, Renjun Pei,