کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230253 | 1470944 | 2017 | 24 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Photoresponsive colorimetric immunoassay based on chitosan modified AgI/TiO2 heterojunction for highly sensitive chloramphenicol detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Photoresponsive colorimetric immunoassay based on chitosan modified AgI/TiO2 heterojunction for highly sensitive chloramphenicol detection Photoresponsive colorimetric immunoassay based on chitosan modified AgI/TiO2 heterojunction for highly sensitive chloramphenicol detection](/preview/png/7230253.png)
چکیده انگلیسی
Chloramphenicol (CAP) is an antibiotic used for a variety of infections, however, it is banned for use in food-producing animals due to its hazards to humans blood. Herein, the highly sensitive photoresponsive colorimetric immunoassay was exploited based on AgI/TiO2 heterojunction nanomaterials. Firstly, chitosan modified AgI/TiO2 photocatalyst (CS-AgI/TiO2) was synthesized by deposition-precipitation method, which was characterized by transmission electron microscope (TEM), scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray powder diffraction (XRD), UV-Vis diffuse reflectance spectroscopy (DRS) and Fourier transform infrared spectroscopy (FTIR). Then, by utilizing CS-AgI/TiO2 labeled CAP antibody as tags, a novel competitive-type immunoassay was constructed for detection of CAP on the surface of CAP-BSA modified magnetic bead. The color development of 3,3â²,5,5â²-tetramethylbenzidine (TMB) was obtained under visible light irradiation (λâ¥420 nm) and the assay was carried out in pH 3.0 ABS buffer solution by UV-Vis spectrophotometry. Under optimal conditions, the absorbance values decreased with the increasing of CAP levels in sample, which exhibited linear in the range of 0.03-12.53 nM with a detection limit of 0.03 nM. Meanwhile, the selectivity, repeatability, and stability were acceptable. Excitedly, no significant difference was observed for detection of the spiked CAP in the real food samples with the referenced values.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 87, 15 January 2017, Pages 579-586
Journal: Biosensors and Bioelectronics - Volume 87, 15 January 2017, Pages 579-586
نویسندگان
Honghong Chang, Jia Lv, Haochun Zhang, Bing Zhang, Wenlong Wei, Yan Qiao,