کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230410 | 1470946 | 2016 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
SERS nanoprobes for the monitoring of endogenous nitric oxide in living cells
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Nitric Oxide (NO) is a significant gaseous signalling molecule in various pathological and physiological pathways, whereas many of its functions are still ambiguous in part because of the shortage of powerful detection approaches. Herein, we present a type of reaction-based surface-enhanced Raman scattering (SERS) nanoprobes, o-phenylenediamine-modified gold nanoparticles (AuNPs/OPD), to detect the level of the endogenous NO in living cells. The detection is achieved through the SERS variation of AuNPs/OPD caused by the reaction between NO and OPD on the surface of AuNPs. The proposed SERS nanoprobes have a good stability and a rapid response to NO within 30Â s Moreover, as a result of the reaction specificity coupled with SERS fingerprinting, AuNPs/OPD nanoprobes demonstrate high selectivity towards NO over other biologically relevant species with a sensitivity at 10â7Â M level. Thereby, this SERS strategy can be used for monitoring NO that is endogenously produced in living macrophages, indicating immense potential in studying NO-involved pathophysiological processes in biological systems.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 85, 15 November 2016, Pages 324-330
Journal: Biosensors and Bioelectronics - Volume 85, 15 November 2016, Pages 324-330
نویسندگان
Jing Cui, Kai Hu, Jia-Jia Sun, Lu-Lu Qu, Da-Wei Li,