کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230531 | 1470946 | 2016 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel nanosensor composed of aptamer bio-dots and gold nanoparticles for determination of thrombin with multiple signals
ترجمه فارسی عنوان
یک نانوسنسور جدید شامل زیست نقطه های آپتامر و نانوذرات طلا برای تعیین ترومبین با سیگنال های متعدد است
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Thrombin is a crucial multifunctional enzyme involved in many physiological and pathological processes. Its detection is of great importance. In this work, a novel bio-dots nanosensor for detection of thrombin with colorimetric, fluorometric and light-scattering signals is developed. This nanosensor is composed of thrombin-binding aptamer bio-dots (TBA-dots) and gold nanoparticles (AuNPs), where TBA-dots serve as fluorometric reporter and AuNPs function as multiple roles as colorimetric reporter, light scattering reporter and fluorescence quencher. TBA-dots retain inherent structures of aptamer to specifically interact with thrombin and simultaneously induce the aggregation of AuNPs. The mechanism of the sensing system is based on distance-dependent aggregation of AuNPs and fluorescence resonance energy transfer (FRET). The nanosensor needs no further surface functionalization required for the as-prepared bio-dots and AuNPs, which provides a sensitive method for the selective detection of thrombin with a detection limit as low as 0.59Â nM. In addition, it provides a brand new perspective for bio-dots and its potential use in bioanalysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 85, 15 November 2016, Pages 798-806
Journal: Biosensors and Bioelectronics - Volume 85, 15 November 2016, Pages 798-806
نویسندگان
Lan Kuang, Shu-Ping Cao, Li Zhang, Qiu-Hong Li, Zhi-Chao Liu, Ru-Ping Liang, Jian-Ding Qiu,