کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230943 | 1470953 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Highly sensitive electrochemical sensing platform for lead ion based on synergetic catalysis of DNAzyme and Au-Pd porous bimetallic nanostructures
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
In this work, a sensitive and specific electrochemical biosensor for lead ion (Pb2+) detection was developed by coupling with synergetic catalysis of porous Au-Pd bimetallic nanoparticles and hemin/G-quadruplex-based DNAzyme. In the presence of target Pb2+, the substrate strand was cleaved and the enzyme strand was released. Subsequently, G-rich DNA-labeled Au-Pd bimetallic nanoparticle was linked with the released enzyme strand through the helper DNA. Upon addition of hemin, a large number of hemin/G-quadruplex-based DNAzyme molecules were formed on the electrode to serve as nicotinamide adenine dinucleotide hydrogen (NADH) oxidase and peroxidase mimics. DNAzyme could catalyzed the reduction of H2O2, generated from NADH in the presence of O2, to produce an electrochemical signal when using thionine as the electron mediator. Introduction of porors Au-Pd bimetallic nanoparticles could enhance the detectable signal and cause the increase in the sensitivity. Experimental results showed that the variations (âI) in the cathodic peak currents of the biosensor were linearly dependent on target Pb2+ concentrations from 1.0Â pM to 100Â nM with a detection limit (LOD) of 0.34Â pM. The excellent performance of the sensing platform indicated its promising prospect as a valuable tool for simple and cost-effective Pb2+ detection in practical application.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 78, 15 April 2016, Pages 236-243
Journal: Biosensors and Bioelectronics - Volume 78, 15 April 2016, Pages 236-243
نویسندگان
Qian Zhou, Youxiu Lin, Yuping Lin, Qiaohua Wei, Guonan Chen, Dianping Tang,