کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
7231195 1470952 2016 6 صفحه PDF سفارش دهید دانلود رایگان
عنوان انگلیسی مقاله ISI
Label-free liquid crystal biosensor for L-histidine: A DNAzyme-based platform for small molecule assay
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موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
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Label-free liquid crystal biosensor for L-histidine: A DNAzyme-based platform for small molecule assay
چکیده انگلیسی
We have developed a novel DNAzyme-based liquid crystal (LC) biosensor with high sensitivity for L-histidine, which is based on L-histidine-mediated formation of DNA duplexes by cleaving DNAzyme using L-histidine, resulting in a remarkable optical signal. Firstly, an optimal amount of capture probe is bound to the glass slide, which changes the surface topology as little as possible and shows a zero-background for the sensing system. When the DNAzyme molecule is cleaved by the target, L-histidine, a partial substrate strand is produced, which in turn can hybridize with the capture probe, forming a DNA duplex. The DNA duplexes induce LC molecules to undergo a homeotropic-to-tiled transition, obtaining a remarkable optical signal. The results show that the DNAzyme-based LC biosensor is highly sensitive to L-histidine with a detection limit of 50 nM. Compared with previously reported multi-step amplified methods, this newly designed assay system for L-histidine has no amplified procedures with comparable sensitivity. This method is an unprecedented example of DNAzyme-based LC biosensor for small molecules, which has potential to offer a DNAzyme-based LC model used in various targets.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 79, 15 May 2016, Pages 650-655
نویسندگان
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