کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7231378 | 1470956 | 2016 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Novel gold nanocluster electrochemiluminescence immunosensors based on nanoporous NiGd-Ni2O3-Gd2O3 alloys
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Herein, three-dimensional nanoporous NiGd alloy (NP-NiGd) was prepared by selectively dealloy Al from NiGdAl alloy in mild alkaline solution, then Ni2O3 and Gd2O3 grew further on the surface of NP-NiGd to obtain the NP-NiGd-Ni2O3-Gd2O3. On this basis, NP-NiGd-Ni2O3-Gd2O3 was further functionalized with gold nanoparticles (NP-NiGd-Ni2O3-Gd2O3@Au) and acted as sensor platform to fabricate a novel electrochemiluminescence (ECL) immunosensor. Bovine serum albumin protected gold nanoclusters (AuNCs@BSA) were prepared and acted as illuminant. AuNCs@BSA modified graphene oxide (GO/AuNCs@BSA) were used as labels of second antibody. In order to characterize the performance of the ECL immunosensor, carcino embryonie antigen (CEA) was used as the model to complete the experiments. Due to the good performances of NP-NiGd-Ni2O3-Gd2O3@Au (high surface area, excellent electron conductivity) and AuNCs@BSA (low toxicity, biocompatibility, easy preparation and good water solubility), the ECL immunosensor exhibited a wide range from 10â4 to 5Â ng/mL with a detection limit of 0.03Â pg/mL (S/N=3). The immunosensor with excellent stability, acceptable repeatability and selectivity provided a promising method to detect CEA in human serum sample sensitively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 75, 15 January 2016, Pages 142-147
Journal: Biosensors and Bioelectronics - Volume 75, 15 January 2016, Pages 142-147
نویسندگان
Xiaohui Lv, Hongmin Ma, Dan Wu, Tao Yan, Lei Ji, Yixin Liu, Xuehui Pang, Bin Du, Qin Wei,