کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7231701 1470954 2016 26 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Switchable fluorescence of gold nanoclusters for probing the activity of alkaline phosphatase and its application in immunoassay
ترجمه فارسی عنوان
فلورسانس قابل تعویض نانوکلاسترهای طلای برای بررسی فعالیت آلکالین فسفاتاز و کاربرد آن در آزمایش ایمنی
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this work, a novel strategy for modulating the fluorescence of gold nanoclusters (Au NCs) is developed. The fluorescence of bovine serum albumin (BSA) protected Au NCs is firstly quenched by KMnO4 and then restored by ascorbic acid (AA) due to the deterioration/restoration of the surface structure. Based on which, a novel “switch-on” fluorescent assay for probing the activity of alkaline phosphatase (ALP) is developed with a detection limit as low as 0.002 U/L. In addition, this testing protocol is also expanded to the detection of the inhibitor of ALP and mouse IgG (as a model), the detection limits are 15 ng/mL for the inhibitor of 2,4-Dichlorophenoxyacetic acid (2,4-DA) and 1.5 pg/mL for mouse IgG. The present method paves a new way to develop convenient, sensitive, and selective metal NCs-based fluorescent “turn-on” probes with promising applications in versatile biosensing.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 77, 15 March 2016, Pages 666-672
نویسندگان
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