کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7231816 | 1470957 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ultrasensitive electrochemical immunosensor based on dual signal amplification process for p16INK4a cervical cancer detection in clinical samples
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Ultrasensitive electrochemical immunosensor based on dual signal amplification process for p16INK4a cervical cancer detection in clinical samples Ultrasensitive electrochemical immunosensor based on dual signal amplification process for p16INK4a cervical cancer detection in clinical samples](/preview/png/7231816.png)
چکیده انگلیسی
This article reports an ultrasensitive electrochemical immunosensor for specific detection of p16 and demonstrates its performance for detection of solubilized p16 protein in cell lysates obtained from patients. Sandwich-based immunoreaction couple with double signal amplification strategy based on catalytic enlargement of particle tag was used for high sensitivity and specificity. The conditions were optimized to create an immunoassay protocol. Disposable screen-printed electrode modified with capture antibodies (Ab1) was selected for further implementation towards point-of-care diagnostics. Small gold nanoparticles (15 nm diameter) conjugated with detection antibodies (Ab2) were found to better serve as a detection label due to limited interference with antigen-antibody interaction. Double signal enhancement was performed by sequential depositions of gold and silver layers. This gave the sensitivity of 1.78 μA mL (ng GST-p16)â1 cmâ2 and detection limit of 1.3 ng mLâ1 for GST-p16 protein which is equivalent to 0.49 ng mLâ1 for p16 protein and 28 cells for HeLa cervical cancer cells. In addition to purified protein, the proposed immunosensor effectively detected elevated p16 level in cervical swab samples obtained from 10 patients with positive result from standard Pap smear test, indicating that an electrochemical immunosensors hold an excellent promise for detection of cervical cancer in clinical setting.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 74, 15 December 2015, Pages 673-679
Journal: Biosensors and Bioelectronics - Volume 74, 15 December 2015, Pages 673-679
نویسندگان
Pattasuda Duangkaew, Satita Tapaneeyakorn, Chayachon Apiwat, Tararaj Dharakul, Somsak Laiwejpithaya, Proespichaya Kanatharana, Rawiwan Laocharoensuk,