کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7231842 | 1470960 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
RNA-regulated molecular tweezers for sensitive fluorescent detection of microRNA from cancer cells
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: RNA-regulated molecular tweezers for sensitive fluorescent detection of microRNA from cancer cells RNA-regulated molecular tweezers for sensitive fluorescent detection of microRNA from cancer cells](/preview/png/7231842.png)
چکیده انگلیسی
We describe here the construction of the DNA self-assembled molecular tweezers and the application of the tweezers for the monitoring of microRNA (miR-141) from human prostate cancer cells. The self-assembly formation of the DNA tweezers and the regulation of the tweezers upon alternative addition of the fuel miR-141 and the anti-fuel strands are characterized by native polyacrylamide gel electrophoresis. The addition of miR-141 to the DNA tweezers turns “off” the tweezers, while subsequent introduction of the anti-fuel strands switches the tweezers back to the “on” state, which verifies the regulatory ability of the tweezers. The miR-141-regulated DNA tweezers are concentration dependent and can be employed for sensitive detection of miR-141 down to 0.6Â pM. The DNA tweezers also show high selectivity toward the fuel strand and can be used to monitor miR-141 expression in cancer cells, which provides new opportunities for the application of the dynamic DNA devices in clinical diagnostics.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 71, 15 September 2015, Pages 98-102
Journal: Biosensors and Bioelectronics - Volume 71, 15 September 2015, Pages 98-102
نویسندگان
Xue Gong, Wenjiao Zhou, Daxiu Li, Yaqin Chai, Yun Xiang, Ruo Yuan,