کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7231902 | 1470961 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A carbon nanofiber-based label free immunosensor for high sensitive detection of recombinant bovine somatotropin
ترجمه فارسی عنوان
یک ایمونوسنزر برچسب ایمنی مبتنی بر نانو فایبر برای تشخیص حساسیت بالا سومااتوتروپین گاوی نوترکیب
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کلمات کلیدی
نانوفیبری کربن، سموتوتروپین گاوهای نوترکیب، بدون برچسب ایمونوسنزر الکتروشیمیایی،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A carbon nanofiber-based label free electrochemical immunosensor for sensitive detection of recombinant bovine somatotropin (rbST) was developed. In this immunosensor design, a mild site-directed antibody immobilization via interaction of boronic acid and oligosaccharide moiety found on Fc region of an antibody was performed to preserve the biological activity of antibody and improve the sensor's sensitivity. Electrochemical characterization of the immunosensor fabrication was carried out by differential pulse voltammetry (DPV) in Fe(CN)63â/Fe(CN)64â probe. A comparison study between different transducer platforms showed carbon nanofiber gave higher current signal response than single-walled carbon nanotube. In this work, calibration curve was obtained from the decrease of DPV peak current of Fe(CN)63â/Fe(CN)64â after immunocomplexed was formed. A linear relationship between DPV current change signal response and rbST concentrations from 1Â pg/mL to 10Â ng/mL (correlation coefficient of 0.9721) was achieved with detection limit of 1Â pg/mL. Our developed immunosensor demonstrated high selectivity in cross-reactivity studies and a good percentage recovery in spiked bovine serum sample.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 70, 15 August 2015, Pages 48-53
Journal: Biosensors and Bioelectronics - Volume 70, 15 August 2015, Pages 48-53
نویسندگان
Syazana Abdullah Lim, Minhaz Uddin Ahmed,