کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7232446 | 1470965 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Electrochemical current rectification-a novel signal amplification strategy for highly sensitive and selective aptamer-based biosensor
ترجمه فارسی عنوان
اصلاح جریان الکتریکی - یک استراتژی تقویت سیگنال جدید برای حسگر و حساسیت سنجی بیوا حسگر مبتنی بر آپتامر است
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کلمات کلیدی
اصلاح جریان الکتروشیمیایی، آپتامر، تقویت سیگنال، بیوسنسور الکتروشیمیایی،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Electrochemical aptamer-based (E-AB) sensors represent an emerging class of recently developed sensors. However, numerous of these sensors are limited by a low surface density of electrode-bound redox-oligonucleotides which are used as probe. Here we propose to use the concept of electrochemical current rectification (ECR) for the enhancement of the redox signal of E-AB sensors. Commonly, the probe-DNA performs a change in conformation during target binding and enables a nonrecurring charge transfer between redox-tag and electrode. In our system, the redox-tag of the probe-DNA is continuously replenished by solution-phase redox molecules. A unidirectional electron transfer from electrode via surface-linked redox-tag to the solution-phase redox molecules arises that efficiently amplifies the current response. Using this robust and straight-forward strategy, the developed sensor showed a substantial signal amplification and consequently improved sensitivity with a calculated detection limit of 114Â nM for ATP, which was improved by one order of magnitude compared with the amplification-free detection and superior to other previous detection results using enzymes or nanomaterials-based signal amplification. To the best of our knowledge, this is the first demonstration of an aptamer-based electrochemical biosensor involving electrochemical rectification, which can be presumably transferred to other biomedical sensor systems.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 66, 15 April 2015, Pages 62-68
Journal: Biosensors and Bioelectronics - Volume 66, 15 April 2015, Pages 62-68
نویسندگان
Lingyan Feng, Arumugam Sivanesan, Zhaozi Lyu, Andreas Offenhäusser, Dirk Mayer,