کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7232632 1644974 2015 20 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
DNA-engineered chiroplasmonic heteropyramids for ultrasensitive detection of mercury ion
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
DNA-engineered chiroplasmonic heteropyramids for ultrasensitive detection of mercury ion
چکیده انگلیسی
In this study, plasmonic heteropyramids (HPs) made from two different sized gold nanoparticles (Au NPs) and five ssDNA sequences and their application for ultrasensitive detection of mercury ion (Hg2+) were demonstrated. Four ssDNA sequences were used as building blocks to form a pyramidal DNA frame, which contains a T-rich probe DNA at one vertex and three sulfhydryl groups modified with 10 nm Au NPs at the other three vertices. Another T-rich DNA sequence was modified and attached to a 25nm Au NP. In the presence of Hg2+ ions, 25 nm Au NPs hybridized with pyramidal DNA frame to build the plasmonic HPs based on T-Hg2+-T interaction, which exhibits unprecedented circular dichroism (CD) signal in the visible region. Based on this mechanism, a simple, high sensitive and selective chiroplasmonic HPs-based probe was constructed and demonstrated for Hg2+ ions detection. Under optimized conditions, Hg2+ ions could be selectively detected in a concentration range from 1 to 500 pg mL−1 with a limit of detection of 0.2 pg mL−1, which is much lower than the strictest Hg2+ safety requirement of 1 ng mL−1 in water.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 68, 15 June 2015, Pages 516-520
نویسندگان
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