کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7232842 | 1470964 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Tailored carbon nanotube immunosensors for the detection of microbial contamination
ترجمه فارسی عنوان
ایمنوسنسورهای نانولوله کربنی برای شناسایی آلودگی میکروبی
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کلمات کلیدی
ایمونواسی الکتروشیمیایی، نانولوله های کربنی، نانوذرات اکسید آهن، بی حرکت سازی آنتی بادی، تشخیص ویروس،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The use of carbon nanotubes (CNTs) as building blocks in the design of electrochemical biosensors has been attracting attention over the last few years, mainly due to their high electrical conductivity and large surface area. Here, we present two approaches based on tailored single-walled CNTs (SWCNTs) architectures to develop immunosensors for the bacteriophage MS2, a virus often detected in sewage-impacted water supplies. In the first approach, SWCNTs were used in the bottom-up design of sensors as antibody immobilization support. Carboxy-functionalised SWCNTs were covalently tethered onto gold electrodes via carbodiimide coupling to cysteamine-modified gold electrodes. These SWCNTs were hydrazide functionalized by electrochemical grafting of diazonium salts. Site-oriented immobilization of antibodies was then carried out through hydrazone bond formation. Results showed microarray electrode behavior, greatly improving the signal-to-noise ratio. Excellent sensitivity and limit of detection (9.3Â pfu/mL and 9.8Â pfu/mL in buffer and in river water, respectively) were achieved, due to the combination of the SWCNTsâ² ability to promote electron transfer reactions with electroactive species at low overpotentials and their high surface-to-volume ratio providing a favorable environment to immobilize biomolecules. In the second approach, SWCNTs were decorated with iron oxide nanoparticles. Diazonium salts were electrochemically grafted on iron-oxide-nanoparticle-decorated SWCNTs to functionalize them with hydrazide groups that facilitate site-directed immobilization of antibodies via hydrazone coupling. These magnetic immunocarriers facilitated MS2 separation and concentration on an electrode surface. This approach minimized non-specific adsorptions and matrix effects and allowed low limits of detection (12Â pfu/mL and 39Â pfu/mL in buffer and in river water, respectively) that could be further decreased by incubating the magnetic immunocarriers with larger volumes of sample. Significantly, both approaches permitted the detection of MS2 to levels regularly encountered in sewage-impacted environments.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 67, 15 May 2015, Pages 642-648
Journal: Biosensors and Bioelectronics - Volume 67, 15 May 2015, Pages 642-648
نویسندگان
B. Prieto-Simón, N.M. Bandaru, C. Saint, N.H. Voelcker,