کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7232855 | 1470965 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A label-free electrochemical aptasensor based on the catalysis of manganese porphyrins for detection of thrombin
ترجمه فارسی عنوان
یک اپتاسنسور الکتروشیمیایی بدون برچسب، بر اساس کاتالیزوری پورفیرین های منگنز برای تشخیص ترومبین
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A novel manganese porphyrin (MnPP)-catalyzed aerobic oxidation of l-cysteine to disulfides (RSSR) was firstly found and applied into electrochemical aptasensor with a label-free technique for signal amplification. The possible catalytic mechanism of the catalytic reaction where MnPP catalyzed l-cysteine with thiol (RSH) structure to RSSR was discussed in detail. For fabrication of the aptasensor, thionine (Thi), which served as an electron mediator, was mixed with MnPP and immobilized on the nafion coated carbon electrode through ion exchange adsorption. Gold nanoparticle (nano-Au) was assembled on the Thi for immobilizing thrombin binding aptamer (TBA). In the presence of thrombin (TB), TBA will capture TB and form TBA-TB composite thus perturbed electron transfer, leading to decrease of the current for quantitatively detecting TB. Under optimal condition, the electrochemical aptasensor exhibited a linear range of 0.1-25Â nM with a detection limit of 0.02Â nM. This work opens a novel way for signal amplification study about porphyrins that served as mimetic enzyme to thiol in electrochemical aptasensor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 66, 15 April 2015, Pages 585-589
Journal: Biosensors and Bioelectronics - Volume 66, 15 April 2015, Pages 585-589
نویسندگان
Yingning Zheng, Yali Yuan, Yaqin Chai, Ruo Yuan,