کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7232871 | 1470967 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Membrane filter-assisted surface enhanced Raman spectroscopy for the rapid detection of E. coli O157:H7 in ground beef
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Membrane filter-assisted surface enhanced Raman spectroscopy for the rapid detection of E. coli O157:H7 in ground beef Membrane filter-assisted surface enhanced Raman spectroscopy for the rapid detection of E. coli O157:H7 in ground beef](/preview/png/7232871.png)
چکیده انگلیسی
Consumption of food contaminated with Escherichia coli O157:H7 is one of the major concerns in ensuring food safety. Techniques that are simple and suitable for fast screening to detect and identify pathogens in the food chain is vital to ensure food safety. In this work, we propose a simple and rapid technique to detect low levels of E. coli O157:H7 using membrane filtration and silver intensification steps in combination with surface enhanced Raman spectroscopy (SERS) analysis. The target bacteria can be captured and separated efficiently by two different antibody bearing nanoparticle complexes (magnetic nanoparticles and gold nanoparticles with a Raman reporter). After centrifugal filtration, the bacteria-nanoparticle complexes left on the filter membrane were localized by silver intensification process before probing by SERS. Extremely low concentration of E. coli O157:H7 (~10Â CFU/mL) could be detected within 1Â h and 3Â h from both pure culture and ground beef samples, respectively. This method can potentially be used as an effective pathogen screening tool for routine monitoring.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 64, 15 February 2015, Pages 171-176
Journal: Biosensors and Bioelectronics - Volume 64, 15 February 2015, Pages 171-176
نویسندگان
Il-Hoon Cho, Pushpak Bhandari, Pradip Patel, Joseph Irudayaraj,