کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7232897 | 1470968 | 2015 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel electrochemical DNA biosensor based on HRP-mimicking hemin/G-quadruplex wrapped GOx nanocomposites as tag for detection of Escherichia coli O157:H7
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A novel sensitive electrochemical DNA biosensor was developed for amperometric detection of Escherichia coli O157:H7 (E. coli O157:H7). The graphene oxide (GOx) was utilized as nanocarrier to immobilize thionine (Thi) and the Au nanoparticles coated SiO2 nanocomposites (Au-SiO2) by electrostatic adsorption and the adsorption among nanomaterials. Then a large amounts of signal DNA (S2) and G-quadruplex were immobilized on the GOx-Thi-Au@SiO2 nanocomposites. Finally, hemin was intercalated into the G-quadruplex to obtain the hemin/G-quadruplex structure as HRP-mimicking DNAzyme. On the basis of the signal amplification strategy of GOx-Thi-Au@SiO2 nanocomposites and DNAzyme, the developed DNA biosensor could respond to 0.01Â nM (S/N=3) with a linear calibration range from 0.02 to 50.0Â nM E. coli O157:H7, which could be well accepted for early clinical detection. The studied system provides new opportunities, and might speed up disease diagnosis, treatment and prevention with pathogen.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 63, 15 January 2015, Pages 1-6
Journal: Biosensors and Bioelectronics - Volume 63, 15 January 2015, Pages 1-6
نویسندگان
Yan Li, Jun Deng, Lichao Fang, Kangkang Yu, Hui Huang, Lili Jiang, Wenbin Liang, Junsong Zheng,