Functionalized graphene as sensitive electrochemical label in target-dependent linkage of split aptasensor for dual detection

We herein report an electrochemical biosensor for highly sensitive and selective detection of ATP and adenosine deaminase activity using functionalized grapheme as efficient electrochemical label. The specific binding of ATP and its aptamer can link the split aptamer modified grapheme and magnetic beads together. After enzyme catalysis and magnetic separation, grapheme material anchored on surface would efficiently facilitate electron transfer acting as electrochemical label, thus produce amplified electrochemical signals. Our work would will shed lights on new diagnostic applications of graphene, such as the design of biodevices for detection of other biomolecules DNA, protein, even cancer cells.