کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7232983 1470968 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrostatic interaction between an enzyme and electrodes in the electric double layer examined in a view of direct electron transfer-type bioelectrocatalysis
ترجمه فارسی عنوان
تعامل الکترواستاتیک بین یک آنزیم و الکترودها در دو لایه الکتریکی مورد بررسی با توجه به بیوالکترو کاتالیزوری نوع انتقال مستقیم الکترون
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Effects of the electrode poential on the activity of an adsorbed enzyme has been examined by using copper efflux oxidase (CueO) as a model enzyme and by monitoring direct electron transfer (DET)-type bioelectrocatalysis of oxygen reduction. CueO adsorbed on bare Au electrodes at around the point of zero charge (Epzc) shows the highest DET activity, and the activity decreases as the adsorption potential (Ead; at which the enzyme adsorbs) is far from Epzc. We propose a model to explain the phenomena in which the electrostatic interaction between the enzyme and electrodes in the electric double layer affects the orientation and the stability of the adsorbed enzyme. The self-assembled monolayer of butanethiol on Au electrodes decreases the electric field in the outside of the inner Helmholtz plane and drastically diminishes the Ead dependence of the DET activity of CueO. When CueO is adsorbed on bare Au electrodes under open circuit potential and then is held at hold potentials (Eho) more positive than Epzc, the DET activity of the CueO rapidly decreases with the hold time. The strong electric field with positive surface charge density on the metallic electrode (σM) leads to fatal denaturation of the adsorbed CueO. Such denaturation effect is not so serious at Eho<
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 63, 15 January 2015, Pages 138-144
نویسندگان
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