کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7233225 1470968 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Highly efficient electrogenerated chemiluminescence quenching of PEI enhanced Ru(bpy)32+ nanocomposite by hemin and Au@CeO2 nanoparticles
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Highly efficient electrogenerated chemiluminescence quenching of PEI enhanced Ru(bpy)32+ nanocomposite by hemin and Au@CeO2 nanoparticles
چکیده انگلیسی
In this work, a new signal amplified strategy based on the quenching effect of hemin and Au nanoparticles decorated CeO2 nanoparticles (Au@CeO2 NPs) for ultrasensitive detection of thrombin (TB) is reported for the first time. Herein, the poly(ethylenimine) (PEI) enhanced Ru(bpy)32+ nanocomposite was implemented by direct chemical polymerization, which could provide the desirable enhanced initial ECL signal. Furthermore, the detection aptamer of thrombin (TBA 2) was immobilized on Au@CeO2 NPs to form TBA 2/Au@CeO2 conjugates. Then, the G-rich DNA of TBA 2 sequence could fold into a G-quadruplex structure to embed hemin to obtain the quenching probe of hemin/TBA 2/Au@CeO2 conjugates. In the presence of target TB, the sandwiched structure could be formed between capture aptamer (TBA 1), TB and hemin/TBA 2/Au@CeO2 conjugates, thereby resulting in a proportional quenching in ECL response with TB, due to the quenching of both hemin and Au@CeO2 NPs. As a result, the signal-off aptasensor showed a wider linear range response from 10−13 to 10−8 M with lower detection limit of 0.03 pM.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 63, 15 January 2015, Pages 392-398
نویسندگان
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