کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7233401 1470968 2015 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A label-free fluorescence strategy for sensitive detection of ATP based on the ligation-triggered super-sandwich
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A label-free fluorescence strategy for sensitive detection of ATP based on the ligation-triggered super-sandwich
چکیده انگلیسی
In this study, a label-free fluorescence strategy for sensitive detection of ATP based on the ligation-triggered super-sandwich is reported. We designed a double-stranded DNA (ds-DNA) probe as the substrate of ATP-dependent ligation. SYBR Green I (SG I), a double-duplex DNA specific dye, was employed as the readout signal. In the absence of ATP, the ligation would not occur and the ds-DNA remained intact. Further, a weak fluorescence could be observed due to the intercalation of SG I into the grooves of the ds-DNA probe. In the presence of ATP, T4 DNA ligase would catalyse the ligation between 3ʹ-OH and 5ʹ-PO4 ends between ds-DNA probes. As a result, more binding sites of the SG I were generated and a fluorescence enhancement was obtained. This method showed a good sensitivity with a detection limit of 200 pM and could perfectly discriminate ATP from its analogs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 63, 15 January 2015, Pages 562-565
نویسندگان
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