کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7233450 1470972 2014 28 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Liquid crystal and gold nanoparticles applied to electrochemical immunosensor for cardiac biomarker
ترجمه فارسی عنوان
کریستال مایع و نانوذرات طلا به سیستم ایمنی بدن الکتروشیمیایی برای بیومارکرهای قلبی اعمال می شود
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A label-free electrochemical immunosensor based on the ionic liquid crystal (E)-1-decyl-4-[(4-decyloxyphenyl)diazenyl]pyridinium bromide (Br-Py) coated on a glassy carbon electrode (GCE) for the quantitative detection of myoglobin (Mb), a cardiac marker for acute myocardial infarction, is reported herein for the first time. The monoclonal anti-myoglobin antibody (ab-Mb) was covalently immobilized using glyoxal on a film of polyethyleneimine-coated gold nanoparticles (AuNP-PEI). The proposed method for Mb detection is based on voltammetric suppression of the Br-Py signal when the immunosensor was incubated with Mb antigen. The electrochemical performance of the Mb immunosensor was studied by electrochemical impedance spectroscopy, and cyclic and square-wave voltammetry. Under the optimal conditions, the proposed immunosensor shows a good linear relationship between the electrochemical inhibition response and the concentration of Mb over the range of 9.96-72.8 ng mL−1 with a detection limit of 6.29 ng mL−1. The results obtained indicate that the proposed immunosensor provides good sensitivity and simple operation for detecting acute myocardial infarction with Mb as a biomarker.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 59, 15 September 2014, Pages 127-133
نویسندگان
, , , , ,