Sensitive quantification of vascular endothelial growth factor (VEGF) using porosity induced hydrogel microspheres

Schematic demonstrating the hydrogel microsphere based VEGF detection system. Initially, capture antibody immobilized microspheres were fabricated in the presence of PEG porogen. Upon incubation in PBS, PEG is removed leaving behind a network of interconnected pores. Increased porosity in turn facilitates enhanced diffusion VEGF and target antibody within the spheres.