کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7234113 | 1470981 | 2013 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Visual detection of prion protein based on color complementarity principle
ترجمه فارسی عنوان
تشخیص بصری پروتئین پریون بر اساس اصل تکمیلی رنگ
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کلمات کلیدی
تجمع، رنگ مکمل، پروتئین پریون، نقاط کوانتومی، تشخیص ویژوال،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Two complementary colors mixed in a proper proportion will produce a neutral color in the color theory. A novel colorimetric method on basis of the color complementarity principle has been well-established to detect recombinant prion protein (rPrP). We found that a colorless solution appeared after mixing orange CdTe quantum dots (QDs) with green-blue malachite green (MG) because of color complementarity. After the addition of rPrP into the mixed solution, the color changed from colorless to green-blue because rPrP could induce the aggregation of QDs, rapidly. And it could be observed by naked eyes. Based on this phenomenon, we developed a simple assay for visual detection of rPrP. At the same time, we obtained excellent correlation between absorption and concentrations of rPrP from 1 nmol Lâ1 to 78 nmol Lâ1 with the limit of detection of 0.3 nmol Lâ1 (3Ï). Moreover, it can be applied to determine rPrP in human serum successfully. Importantly, this assay possesses the advantages of simplicity, rapidity, sensitivity, and selectivity, and shows the potential in the clinical diagnostic test of early prion disease and provides the possibility of preventing the spread of prion diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 50, 15 December 2013, Pages 14-18
Journal: Biosensors and Bioelectronics - Volume 50, 15 December 2013, Pages 14-18
نویسندگان
Liping Liang, Yijuan Long, Haijie Zhang, Qinlong Wang, Xiaoxiao Huang, Rui Zhu, Ping Teng, Xiliang Wang, Huzhi Zheng,