Ultrasensitive electrochemical strategy for trace detection of APE-1 via triple signal amplification strategy

► An ultrasensitive electrochemical method is developed for detection of APE-1. ► A triple signal amplification strategy was employed to enhance the sensitivity. ► The porous organic material (PTC-NH2) as electrode modifier could lower the background current. ► The proposed immunosensor shows high sensitivity, specificity and acceptable stability.