Detection and imaging the expression of the trans-membrane protein CD44 in RT112 cells by use of enzyme-labeled antibodies and SECM.

► RT112 cells in patterns on glass expressed the trans-membrane protein CD44. ► Expression of CD44 by the cells lead to immobilisation of enzyme-labeled antibodies. ► Enzymatic reactions allowed imaging of cells by scanning electrochemical microscopy. ► Patterns of RT112 cells assembled and their expression of the target protein imaged. ► Control samples showed minimal activity