A method for fluorescence sensing of adenosine and alkaline phosphatase based on the inhibition of S-adenosylhomocysteine hydrolase activity

► We propose the possible reaction mechanism between NDA and homocysteine. ► This probe discriminates adenosine from a mixture of adenosine analogs. ► This probe can be applied for the detection of adenosine in urine. ► This probe monitors the activity and inhibition of alkaline phosphatases.