Ru(II) encapsulated phosphorylate-terminated silica nanoparticles-based electrochemiluminescent strategy for label-free assay of protein kinase activity and inhibition

► We develop an electrochemiluminescent strategy for assay PKA activity and inhibition. ► The electrochemiluminescent assay is based on Zr4+ and R-PSiNPs. ► Signal amplification of Ru(II) doped phosphorylate-terminated silica nanoparticles. ► It is a simple, sensitive, selective, and universal platform for kinases activity assay.