A non-aggregation colorimetric assay for thrombin based on catalytic properties of silver nanoparticles

- An AgNP-based non-aggregation colorimetric aptasensor was first developed.
- The colorimetric principle was based on AgNP-catalyzed reductive degradation of RhB.
- This assay combined magnetic separation with nanocatalytic amplification.
- The detection limit of thrombin was as low as 0.2 nM with excellent specificity.