Automation of dimethylation after guanidination labeling chemistry and its compatibility with common buffers and surfactants for mass spectrometry-based shotgun quantitative proteome analysis

- Dimethylation after guanidination (2MEGA) uses inexpensive reagents for isotopic labeling of peptides.
- 2MEGA can be optimized and automated for labeling peptides with high efficiency.
- 2MEGA is compatible with several commonly used cell lysis and protein solubilization reagents.
- The automated 2MEGA labeling method can be used to handle a variety of protein samples for relative proteome quantification.