On-chip multiplexed solid-phase nucleic acid hybridization assay using spatial profiles of immobilized quantum dots and fluorescence resonance energy transfer

- Solid-phase multiplexed QD-FRET nucleic acid assay in electrokinetic fluidic chip.
- Concurrent detection of two oligonucleotides based on channel length coverage.
- Selection of “turn-on” and “turn-off” signals from two acceptor dyes and two colors of immobilized QDs, respectively.
- No loss in assay sensitivity when implementing multiplexed assay format.