Cytotoxicity assessment based on the AUC50 using multi-concentration time-dependent cellular response curves

► Dose- and time-dependent cellular responses are used to evaluate the cytotoxicity. ► The CI can reflect the cell number, cell viability and morphological change, etc. ► AUC is more relevant to the intensity of the cell treatment. ► AUC50 can be used for cytotoxicity assessment. ► AUC50 combined with RTCA HT assay can achieve a high-throughput screening.