The hybrid experimental simplex algorithm - An alternative method for 'sweet spot' identification in early bioprocess development: Case studies in ion exchange chromatography

► A novel simplex method best suited to coarsely gridded data was developed. ► This is used for 'sweet spot' identification in two bioprocess development studies. ► The method returns comparable experimental costs to those from DoE methodologies. ► The method is a viable alternative for 'sweet spot' identification in scouting studies.