کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7557539 1491344 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Multiplex single molecule counting technology used to generate interleukin 4, interleukin 6, and interleukin 10 reference limits
ترجمه فارسی عنوان
تکنولوژی شمارش مولکول تک چندگانه که برای تولید اینترلوکین 4، اینترلوکین 6 و اینترلوکین 10 استفاده می شود
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Detecting biomarkers at pg/ml concentrations or below is, in many situations, critical for quantifying levels in healthy individuals as well as the changes that can occur in the progression of disease states. The ability to detect multiple biomarkers from the same sample allows for better diagnoses, more efficient testing, and lower volumes of sample required. Based on single molecule counting technology, a multiplex instrument was designed and built that is capable of detecting cytokines and other low-abundance proteins at sub-pg/ml quantities in human plasma samples. The multiplex single molecule counting instrument was used to generate 95% reference limits for interleukin 4 (IL-4, <0.61 pg/ml), interleukin 6 (IL-6, <6.53 pg/ml), and interleukin 10 (IL-10, <1.08 pg/ml) from 100 healthy human donor plasma samples, with more than 90% of IL-4 concentrations and 100% of IL-6 and IL-10 concentrations above the limit of detection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 503, 15 June 2016, Pages 11-20
نویسندگان
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