کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7584320 | 1492023 | 2018 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Electrochemical DNA biosensor for potential carcinogen detection in food sample
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کلمات کلیدی
Ammonium acetate (PubChem CID: 517165)Ethanol (PubChem CID: 702) - اتانول (PubChem CID: 702)Acetic acid (PubChem CID: 176) - اسید استیک (PubChem CID: 176)Sulfuric acid (PubChem CID: 1118) - اسید سولفوریک (PubChem CID: 1118)Electrochemical biosensor - بیوسنسور الکتروشیمیایی یا زیست حسگرهای الکتروشیمیایی Carcinogens - سرطان زاToxicity - سمی بودنGold nanoparticles - نانوذرات طلاSilica nanospheres - نانوسپیر سیلیکاPotassium dihydrogen phosphate (Pubchem CID: 516951) - پتاسیم دی هیدروژن فسفات (Pubchem CID: 516951)Potassium chloride (PubChem CID: 4873) - کلرید پتاسیم (PubChem CID: 4873)Glutaraldehyde (Pubchem CID: 3485) - گلوتارالدئید (Pubchem CID: 3485)
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The presence of carcinogens in food is a major food safety concern. A nanocomposite-based electrochemical DNA biosensor was constructed for potential carcinogen detection in food samples by immobilizing amine terminated single stranded DNA onto silica nanospheres deposited onto a screen-printed electrode modified using gold nanoparticles. The effect of three different DNA sequences: 15-base guanine, 24-base guanine and 24-base adenine-thymine rich DNA on carcinogen (formaldehyde and acrylamide) detection was evaluated. The competitive binding of the DNA with the carcinogen and electroactive indicator, Methylene blue (MB) was measured using differential pulse voltammetry. Optimization studies were conducted for MB concentration and accumulation time, DNA concentration, buffer concentration, pH and ionic strength. Overall, the 24-base guanine rich DNA yielded the best results with a detection limit of 0.0001â¯ppm, linear range between 0.0001â¯ppm and 0.1â¯ppm and reproducibility below 5% R.S.D. Finally, the results obtained using the biosensor were validated using Ames test.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 269, 15 December 2018, Pages 503-510
Journal: Food Chemistry - Volume 269, 15 December 2018, Pages 503-510
نویسندگان
Nor Diyana Md. Sani, Lee Yook Heng, Radha Swathe Priya Malon Marugan, Nor Fadilah Rajab,