کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7584350 | 1492023 | 2018 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Digestive aspartic proteases from sábalo (Prochilodus lineatus): Characterization and application for collagen extraction
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کلمات کلیدی
Ethylenediaminetetraacetic acid (PubChem CID: 6049)Acidic proteaseAcetic acid (PubChem CID: 176) - اسید استیک (PubChem CID: 176)Hydrochloric acid (PubChem CID: 313) - اسید هیدروکلریک (PubChem CID: 313)Sodium dodecyl sulfate (PubChem CID: 3423265) - سدیم داودسیل سولفات (PubChem CID: 3423265)Methanol (PubChem CID: 887) - متانول (PubChem CID: 887)Stomach - معده یا شکمSodium hydroxide (PubChem CID: 14798) - هیدروکسید سدیم (PubChem CID: 14798)Collagen - کلاژنSodium chloride (PubChem CID: 5234) - کلرید سدیم (PubChem CID: 5234)Glycine (PubChem CID: 750) - گلیسین (PubChem CID: 750)
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Acid proteases from sábalo stomach mucosa were recovered using salting-out procedure. This single step produced an enzyme extract purified 1.8-fold over the crude extract with a recovery of 45.1% of its initial proteolytic activity. Sábalo proteases exhibited the highest activity at 45â¯Â°C-pH 2.0, showed pH stability between 2.0 and 5.0 and retained more than 70% of its activity after incubation at pH 7.0 for 2â¯h. Fish extract was unstable at temperatures greater than 45â¯Â°C. Its activity was inhibited by pepstatin A but not by PMSF, while EDTA and SDS showed partial inhibitory effects. Presence of CaCl2 and MgCl2 increased the proteolytic activity, while increasing concentrations of NaCl strongly decreased it. In addition, compared to the acid extraction method, the use of sábalo enzymatic extract increased 1.7 times the yield of collagen extraction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 269, 15 December 2018, Pages 610-617
Journal: Food Chemistry - Volume 269, 15 December 2018, Pages 610-617
نویسندگان
Antonella Valeria Acevedo Gomez, Gabriela Gomez, Ester Chamorro, Soledad Bustillo, Laura Cristina Leiva,