کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7584776 1492030 2018 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid identification of Staphylococcus aureus, Vibrio parahaemolyticus and Shigella sonnei in foods by solid phase microextraction coupled with gas chromatography-mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Rapid identification of Staphylococcus aureus, Vibrio parahaemolyticus and Shigella sonnei in foods by solid phase microextraction coupled with gas chromatography-mass spectrometry
چکیده انگلیسی
A novel approach for rapid identification of three foodborne pathogens including Staphylococcus aureus, Vibrio parahaemolyticus and Shigella sonnei in foods by solid phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS) was established. After cultivation 24, 18 and 20 h for Staphylococcus aureus, Vibrio parahaemolyticus and Shigella sonnei, respectively, the microbial volatile organic compounds (MVOCs) were extracted with a SPME device equipped with divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) coated fibers. The DB-1701P column was applied for separation of MVOCs. A total of 17, 13 and 14 volatile organic compounds were identified as characteristic MVOCs of Staphylococcus aureus, Vibrio parahaemolyticus and Shigella sonnei, respectively. Similarity of the MVOC chromatographic fingerprints for the bacteria were calculated and compared, and the results showed that the established method is stable, reproducible, accurate and has the potential to identify the three bacteria in food samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 262, 1 October 2018, Pages 7-13
نویسندگان
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