کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7585481 | 1492039 | 2018 | 39 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The antioxidant activity and genotoxicity of isogarcinol
ترجمه فارسی عنوان
فعالیت آنتی اکسیدان و ژنوتوکسین ایزوگارسینول
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The antioxidant activity and genotoxicity of isogarcinol were assessed by several in vitro tests. Its IC50 values for DPPH and ABTS were 36.3â¯Â±â¯3.35â¯ÂµM and 16.6â¯Â±â¯3.98â¯ÂµM, respectively, which were all lower than those of VC and BHT. Isogarcinol had no cyctotoxic or promotional activities at 1-10â¯ÂµM in the CCK-8 assay, and negligible genotoxic effects at 50-500â¯ÂµM on HepG2 cells by the single-cell gel electrophoresis assay. Pre-incubation of the cells with 0.5-1.5â¯ÂµM isogarcinol, before exposure to H2O2, significantly increased cell viability in a concentration-dependent manner. Isogarcinol also reduced intercellular reactive oxygen species accumulation, lactate dehydrogenase release and malondialdehyde levels, and increased superoxide dismutase activity and glutathione levels. Western-blot analysis revealed that it up-regulated pro-caspase-3 and reduced cleaved caspase-3 during H2O2-induced oxidative stress. All the above results indicate that isogarcinol promises to be useful as a natural antioxidant.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 253, 1 July 2018, Pages 5-12
Journal: Food Chemistry - Volume 253, 1 July 2018, Pages 5-12
نویسندگان
Zijin Liu, Gang Li, Cheng Long, Jing Xu, Juren Cen, Xiaobo Yang,