کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7589645 | 1492091 | 2016 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous quantification of sulforaphene and sulforaphane by reverse phase HPLC and their content in Raphanus sativus L. var. caudatus Alef extracts
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
A simple, rapid and precise HPLC assay was developed for the well-known anti-cancer isothiocyanates-sulforaphene (SE) and sulforaphane (SF). The analytical system comprised RP-C18 column with isocratic 5% THF-95% water. High resolution was obtained (and eluted) of two distinct HPLC peaks of similar structures SE and SF analytes (at 23.01 ± 0.02 and 25.65 ± 0.03 min, respectively). The respective LOD vs. LOQ for SE and SF was 0.34 and 0.36 μg/ml vs. 1.02 and 1.08 μg/ml. This assay had the best linearity and accuracy. The recoveries were in the range of 96.83-101.17%. SF and SE were quantified in the pod of Raphanus sativus L. var. caudatus Alef extracts (2253.05 ± 246.18 and 111.94 ± 16.49 μg/g in the crude extract, respectively), while only SE was detected in the stem (1105.14 ± 243.10 μg/g crude extract), as SF was lower than the detection limit. The validated method thus minimized and expedited simultaneous SE and SF analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 201, 15 June 2016, Pages 139-144
Journal: Food Chemistry - Volume 201, 15 June 2016, Pages 139-144
نویسندگان
Sarita Sangthong, Natthida Weerapreeyakul,