کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7589645 1492091 2016 23 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous quantification of sulforaphene and sulforaphane by reverse phase HPLC and their content in Raphanus sativus L. var. caudatus Alef extracts
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Simultaneous quantification of sulforaphene and sulforaphane by reverse phase HPLC and their content in Raphanus sativus L. var. caudatus Alef extracts
چکیده انگلیسی
A simple, rapid and precise HPLC assay was developed for the well-known anti-cancer isothiocyanates-sulforaphene (SE) and sulforaphane (SF). The analytical system comprised RP-C18 column with isocratic 5% THF-95% water. High resolution was obtained (and eluted) of two distinct HPLC peaks of similar structures SE and SF analytes (at 23.01 ± 0.02 and 25.65 ± 0.03 min, respectively). The respective LOD vs. LOQ for SE and SF was 0.34 and 0.36 μg/ml vs. 1.02 and 1.08 μg/ml. This assay had the best linearity and accuracy. The recoveries were in the range of 96.83-101.17%. SF and SE were quantified in the pod of Raphanus sativus L. var. caudatus Alef extracts (2253.05 ± 246.18 and 111.94 ± 16.49 μg/g in the crude extract, respectively), while only SE was detected in the stem (1105.14 ± 243.10 μg/g crude extract), as SF was lower than the detection limit. The validated method thus minimized and expedited simultaneous SE and SF analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 201, 15 June 2016, Pages 139-144
نویسندگان
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