کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7593513 1492118 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A high fat, high cholesterol diet leads to changes in metabolite patterns in pigs - A metabolomic study
ترجمه فارسی عنوان
یک رژیم غذایی با چربی بالا و کلسترول بالا باعث تغییرات در الگوهای متابولیتی در خوک می شود - یک مطالعه متابولیکی
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Non-targeted metabolite profiling can identify biological markers of dietary exposure that lead to a better understanding of interactions between diet and health. In this study, pigs were used as an animal model to discover changes in metabolic profiles between regular basal and high fat/high cholesterol diets. Extracts of plasma, fecal and urine samples from pigs fed high fat or basal regular diets for 11 weeks were analysed using ultra-high performance liquid chromatography with high-resolution mass spectrometry (UHPLC-HRMS) and chemometric analysis. Cloud plots from XCMS online were used for class separation of the most discriminatory metabolites. The major metabolites contributing to the discrimination were identified as bile acids (BAs), lipid metabolites, fatty acids, amino acids and phosphatidic acid (PAs), phosphatidylglycerol (PGs), glycerophospholipids (PI), phosphatidylcholines (PCs) and tripeptides. These results suggest the developed approach can be used to identify biomarkers associated with specific feeding diets and possible metabolic disorders related to diet.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 173, 15 April 2015, Pages 171-178
نویسندگان
, , , , , , , ,