کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7595215 | 1492122 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fast simultaneous determination of prominent polyphenols in vegetables and fruits by reversed phase liquid chromatography using a fused-core column
ترجمه فارسی عنوان
تعیین سریع پلی فنول های برجسته در سبزیجات و میوه ها با استفاده از کروماتوگرافی مایع فاز برگشت داده شده با استفاده از ستون هسته
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A reversed-phase high-performance liquid chromatography method with photodiode array detection has been developed enabling the joint determination of 17 prominent flavonoids and phenolic acids in vegetables and fruits. A multi-segmented gradient program using a fused-core column for the separation of several phenolic classes (phenolic acids and flavonoids) has been optimised. The influence of extraction conditions (sample freeze-drying, ultrasound extraction, solvent composition and extraction time) has been also optimised using response surface methodology with tomato samples as a model. Complete recoveries (76-108%) were obtained for the phenolic compounds present in tomato. The developed method provided satisfactory repeatability in terms of peak area (RSD < 2.9%) and retention time (RSD < 0.2%) both for standards and real samples. Detection limits ranged between 3 and 44 μg kgâ1 for the detected polyphenols. This method is recommended for routine analysis of large number of samples typical of production quality systems or plant breeding programs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 169, 15 February 2015, Pages 169-179
Journal: Food Chemistry - Volume 169, 15 February 2015, Pages 169-179
نویسندگان
Raúl MartÃ, Mercedes Valcárcel, José Manuel Herrero-MartÃnez, Jaime Cebolla-Cornejo, Salvador Roselló,