کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7596696 | 1492128 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Structure and property modification of an oligochitosan-glycosylated and crosslinked soybean protein generated by microbial transglutaminase
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The impacts of oligochitosan glycosylation and crosslinking on the structure and properties of a soybean protein were investigated. The reaction was carried out by transglutaminase with 10 kU kgâ1 protein at pH 7.5 and 37 °C for 3 h, under a protein content of 40 g lâ1 and a molar ratio of acyl donor to oligochitosan acceptor of 1:3. The modified protein contained glucosamine at a concentration of 12.1 g kgâ1 protein. Electrophoresis and infrared spectroscopy analysis confirmed the modified protein to be crosslinked and glycosylated. Circular dichroism analysis showed the modified protein possessed a decreased α-helix and β-structure. The modified protein exhibited lower surface hydrophobicity and emulsifying activity but higher emulsion stability than the soybean protein, it also had better water and oil binding capacity (12.2 g and 3.5 ml gâ1 protein, respectively), and could form a thicker protein outer layer in the oil droplets in emulsion. Transglutaminase-induced oligochitosan glycosylation and crosslinking is thus able to modify soybean proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 163, 15 November 2014, Pages 114-119
Journal: Food Chemistry - Volume 163, 15 November 2014, Pages 114-119
نویسندگان
Chun-Li Song, Xin-Huai Zhao,