Effects of enzymatic treatment using Response Surface Methodology on the quality of bread flour

Flour with low α-amylase activity needs to be supplemented with additional α-amylase, but α-amylase added to weak flour can lead to decreased quality of the dough. The objective of this study, was to evaluate the effects of glucose oxidase (1-5/100 g flour) and xylanase (1-3/100 g flour) on the quality of bread flour after optimisation by additions of α-amylase. The effects of enzyme additions on dough properties and bread quality parameters such as specific volume, shape, texture and sensory attributes were determined by Response Surface Methodology (RSM) using a central composite design. Results of RSM modelling showed that glucose oxidase and xylanase improved the quality of bread and dough but effects were dose dependent. In this work, the optimal dose of glucose oxidase and xylanase were (30 and 20) ppm, respectively.