کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7599916 1492144 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of an indirect competitive immunoassay for walnut protein component in food
ترجمه فارسی عنوان
توسعه یک آزمایش ایمنی رقابتی غیرمستقیم برای جزء پروتئین گردو در غذا
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Among food allergens, walnut is a frequent cause of adverse food reactions in allergic patients. In this study, the walnut allergen protein 2S albumin precursor (Jug r 1) cDNA was synthesised and cloned into the pGEX-6P-1 expression vector. The recombinant plasmids were transformed into Escherichia coli (E. coli) BL21(DE3) pLys for expression of protein Jug r 1. Polyclonal antibodies were prepared against the expressed purified Jug r 1 protein. An indirect competitive enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of walnut soluble proteins in processed foods was developed using the prepared polyclonal antibodies. The developed ELISA had a high specificity, walnut protein standard solution at 2.2 ng/mL [inhibition concentration (IC80) of the competitive test] was clearly identified by the ELISA. The mean recoveries ranged from 86% to 112%. The coefficient of variation (CV) for the 4 model foods was 6.4-8.7%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 147, 15 March 2014, Pages 106-110
نویسندگان
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