کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7607476 | 1493359 | 2018 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of radial and axial heterogeneities of chromatographic columns by flow reversal
ترجمه فارسی عنوان
تعریف ناهمگونی های شعاعی و محوری ستون های کروماتوگرافی با تغییر جریان
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کلمات کلیدی
بسته بندی ستون، ساختار تخت، ناهمگونی ستون شعاعی و محوری، تکنیک بازگشت معکوس، تمرکز باند، ستون قطری بی نهایت،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The impact of the column length (5, 10, and 15â¯cm) and packing mode (constant pressure and constant flow rate up to 15,000â¯psi) on the radial and axial heterogeneities of 3.0â¯mm i.d. research prototype columns packed with the same batch of 2.4â¯Î¼m BEH-C18 particles was investigated by the flow reversal technique. The data were gathered for a non-retained marker (uracil, acetonitrile/water eluent mixture, 80/20, v/v, flow rate 0.5â¯mL/min, Tâ¯=â¯297â¯K) and revealed that the radial heterogeneity of the packed bed, characterized by the center-to-wall relative velocity bias (Ïβ) and its length scale, is nearly independent on the packing mode: the velocity biases extend over a same length scale estimated at 154â¯Î¼m while Ïβ is in between 4% and 6% for all columns. Secondly, the data revealed that the column length has a slight impact on Ïβ: assuming a two-region (wall and center regions) stochastic model of transcolumn eddy dispersion, Ïβ increases from 4.6% to 5.1% and to 6.1% for 5, 10, and 15â¯cm long columns, respectively, when packed at constant flow rate. For columns packed at constant pressure, Ïβ increases from 5.0% to 5.2% and to 5.6%, respectively. Finally, it is found that all columns are axially heterogeneous: the bottom half, which is packed first (column inlet), is slightly more uniform than the top half (column outlet) which is packed last. Overall, the results of the flow reversal experiments corroborate recent observations (130â¯Î¼m thick wall region and Ïβâ¯=â¯5.0%) based on flow simulations in a focused-ion-beam scanning electron microscopy (FIB-SEM) based 3D reconstruction from a 2.1â¯mmâ¯Ãâ¯50â¯mm column packed with 2â¯Î¼m BEH-C18 particles.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1567, 14 September 2018, Pages 164-176
Journal: Journal of Chromatography A - Volume 1567, 14 September 2018, Pages 164-176
نویسندگان
Fabrice Gritti, Mike Dion, Attila Felinger, Mike Savaria,