کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7607590 | 1493362 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mixed-mode ion exchange-based integrated proteomics technology for fast and deep plasma proteome profiling
ترجمه فارسی عنوان
تکنولوژی پروتئومیک یکپارچه یونی مبادله ای برای پروفیل پروتئوم پلاسما سریع و عمیق
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کلمات کلیدی
بیومارکر، پروتئومیک پلاسما، آماده سازی یکپارچه نمونه، تجزیه چند بعدی،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Plasma proteome profiling by LC-MS based proteomics has drawn great attention recently for biomarker discovery from blood liquid biopsy. Due to standard multi-step sample preparation could potentially cause plasma protein degradation and analysis variation, integrated proteomics sample preparation technologies became promising solution towards this end. Here, we developed a fully integrated proteomics sample preparation technology for both fast and deep plasma proteome profiling under its native pH. All the sample preparation steps, including protein digestion and two-dimensional fractionation by both mixed-mode ion exchange and high-pH reversed phase mechanism were integrated into one spintip device for the first time. The mixed-mode ion exchange beads design achieved the sample loading at neutral pH and protein digestion within 30â¯min. Potential sample loss and protein degradation by pH changing could be voided. 1â¯Î¼L of plasma sample with depletion of high abundant proteins was processed by the developed technology with 12 equally distributed fractions and analyzed with 12â¯h of LC-MS gradient time, resulting in the identification of 862 proteins. The combination of the Mixed-mode-SISPROT and data-independent MS method achieved fast plasma proteome profiling in 2â¯h with high identification overlap and quantification precision for a proof-of-concept study of plasma samples from 5 healthy donors. We expect that the Mixed-mode-SISPROT become a generally applicable sample preparation technology for clinical oriented plasma proteome profiling.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1564, 24 August 2018, Pages 76-84
Journal: Journal of Chromatography A - Volume 1564, 24 August 2018, Pages 76-84
نویسندگان
Xue Lu, Lin Lin, Wenbin Zhou, Wendong Chen, Jun Tang, Xiujie Sun, Peiwu Huang, Ruijun Tian,