کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7607938 1493368 2018 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitation of isoprenoids for natural rubber biosynthesis in natural rubber latex by liquid chromatography with tandem mass spectrometry
ترجمه فارسی عنوان
شمارش ایزوپرونگید ها برای بیوسنتز لاستیک طبیعی در لاتکس لاستیک طبیعی با استفاده از کروماتوگرافی مایع و اسپکترومتری توده دو طرفه
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The natural rubber molecule is one of the end products of isoprenoids metabolism in the plant. Dimethylallyl diphosphate (DMAPP) and farnesyl pyrophosphate (FPP) are two typical isoprenoids which control the rate of biosynthesis and the molecular weight of natural rubber. A rapid, nonradioactive method for quantitation of DMAPP and FPP in natural rubber latex by liquid chromatography tandem with mass spectrometry (LC-MS/MS) was reported. DMAPP and FPP were determined in the multiple reaction monitoring mode(MRM)followed by separation with a silica-based C18 column. The external standard quantitative method was established, and the results showed limits of quantitation (LOQs) were 28 ng/ml and 33 ng/ml for DMAPP and FPP, respectively. The concentrations were detected 70-96 ng/ml and 242-375 ng/ml for these two isoprenoids in natural rubber latex. Recoveries of the method were in the range 81-93%. Daytime comparison experiments found that FPP had better stability than DMAPP.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1558, 13 July 2018, Pages 115-119
نویسندگان
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