کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7608080 1493372 2018 20 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A study of column equilibration time in hydrophilic interaction chromatography
ترجمه فارسی عنوان
مطالعه زمان همبستگی ستون در کروماتوگرافی تعامل هیدرولیکی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The time taken to achieve full column equilibration for isocratic analysis of acidic, basic, and neutral solutes in hydrophilic interaction liquid chromatography (HILIC) was compared using the mobile phase disturbance method, column efficiency measurements and retention time stability. Full equilibration, which could take up to an hour, was best measured by the last procedure and was found to depend on the nature of the stationary phase, the pre-equilibrium (e.g. storage) solvent and the flow rate. While longer equilibration times are a relatively minor inconvenience in isocratic analysis, they are surprisingly not a barrier to the use of gradient elution in HILIC. A repeatable partial equilibration giving retention time stability equivalent to that in isocratic analysis was demonstrated for an equilibration time of only ∼5 min, using as few as 2 preliminary conditioning runs on a column that had taken the longest time to achieve full equilibration. Due to selectivity changes that occur dependent on the equilibration time, it is necessary to use identical gradient conditions in a series of analyses, which however appears to be facile on a modern HPLC instrument.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1554, 15 June 2018, Pages 61-70
نویسندگان
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